Transfection of the nm23-H1 gene into BcaCD885 cell line inhibits the potential of invasion, adhesion and mobility
Chen, S.-w.; Wen, Y.-m.; Li, L.-j.; Pan, J.; Wang, C.-m.; Liao, X.-l.
Zhonghua Kou Qiang Yi Xue Za Zhi 38(1): 16-19
2003
ISSN/ISBN: 1002-0098 PMID: 12760769 Document Number: 554552
To transfect nm23-H1 into the BcaCD885 cell lines in order to get safe high-efficiency and low-toxicity, and to find out whether nm23-H1 could affect the invasion and metastases ability of BcaCD885 cell lines. Lipofect was used to transfect nm23-H1 into BcaCD885 cell lines; immunohistochemistry was used to detect the difference expression of nm23-H1 between transfected and non-transfected cell lines; then transwell-room and wash way were used to detect the difference of invasion and metastases ability between transfected and non-transfected cell lines. PCMV-NEO-BAM system gave the stability expression of nm23-H1; there was significant different NDPKA expression between transfected and non-transfected BcaCD885 cell lines; the invasion and metastases ability of transfected BcaCD885 cell lines decreased obviously. nm23-H1 can inhibit the metastases of BcaCD885 cell lines significantly.