Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide
Li, S.; Cong, B.; Yan, Y.; Yao, Y.; Ma, C.; Ling, Y.
Chinese Medical Journal 115(2): 276-279
2002
ISSN/ISBN: 0366-6999 PMID: 11940348 Document Number: 551978
Obejective: To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysacchride (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro. Methods: PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA. Results: CCK-8, at concentrations of 10-7 mol/L and 10-6 mol/L, significantly inhibited the expression of mCD14. Release of sCID14 and TNF-alpha in the supernatant was up-regulated by LPS (1 mug/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide. Conclusion: CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.