Effects of zinc on bone development in mouse embryo in vitro
Yu, Z.; Li, Y.; Zheng, A.
Zhonghua Yu Fang Yi Xue Za Zhi 36(2): 97-99
2002
ISSN/ISBN: 0253-9624 PMID: 12410961 Document Number: 551134
Objective This study was designed to explore the effects of zinc on bone development. Methods Forelimbs of mice with 16 d gestation were cultured by a rotating device. Results Contents of OC and 45Ca and activities of AKP in the bone tissues cultured at zinc-deficiency media and at media with 120 mumol/L of Zn2+ decreased significantly. Synthesis of OC, absorption of calcium and activities of AKP in the bone tissues cultures at media with 45 and 70 mumol/L of Zn2+ increased significantly. Radiograph of bone tissues showed that length of long bone cultured at zinc-deficiency media and at media with 120 mumol/L of Zn2+ shortened and their density reduced, and those cultured at media with 45 and 70 mumol/L of zinc increased, as compared with self-control bone. Histological analysis showed the death of bone cells and loss of stroma in the bone tissues cultured at media with 120 mumol/L of Zn2+, and active proliferation and differentiation of bone cells, and secretion and synthesis of osteoid increased in the bone tissues cultured at media with 45 and 70 mumol/L of zinc. Conclusions Adequate supplementation of zinc could promote formation and development of bone tissues and deficiency or excess of zinc could alter their growth and development and normal metabolism.