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HER-2/neu evaluation by immunohistochemistry and fluorescence in situ hybridization in breast cancer: implications for daily laboratory practice

Larsimont, D.; Di Leo, A.; Rouas, G.; Paesmans, M.; Ferreira-Filho, F.; Bernard, C.; Cardoso, F.; Verhest, A.; Piccart, M.J.; Gancberg, D.

Anticancer Research 22(4): 2485-2490

2002

ISSN/ISBN: 0250-7005
PMID: 12174949
Document Number: 549904
Background: HER-2/neu status was determined by immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH) methods in more than 300 paraffin-embedded primary breast cancer samples. Materials and Methods: HER-2/neu status was determined by FISH using the PathVysion kit (Vysis) and by IHC using either a monoclonal antibody CB11 or a cocktail of antibodies: the monoclonal TAB250 and the polyclonal pAb1. Results: Of the 324 cases evaluable by IHC, 65 out of 318 (20%) and 24 out of 324 (7%) were scored as positive when using the antibody cocktail and the CB11, respectively. HER-2/neu gene amplification occurred in 64 out of 324 cases (20%). Concordance of FISH and IHC was found in 285 out of 318 cases (90%) and 278 out of 324 cases (86%) using the cocktail and the CB11, respectively. Conclusion: The cost-effectiveness analysis revealed that the use of a sensitive IHC method followed by confirmation of positive results by FISH considerably decreased the FISH costs and may become standard practice for HER-2/neu evaluation.

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Li, H.-H.; Ma, F.; Zeng, X.; Wang, J.-Y.; Yuan, P.; Fan, Y.; Xu, B.-H. 2011: Comparison of fluorescence in situ hybridization and immunohistochemistry assessment for Her-2 status in breast cancer and its relationship to clinicopathological characteristics Zhonghua Yi Xue Za Zhi 91(2): 76-80
Bouchalová, K.; Trojanec, R.; Kolár, Z.; Cwiertka, K.; Cernáková, I.; Mihál, V.; Hajdúch, M. 2006: Analysis of ERBB2 and TOP2A gene status using fluorescence in situ hybridization versus immunohistochemistry in localized breast cancer Neoplasma 53(5): 393-401
Kounelis, S.; Kapranos, N.; Malamos, N.; Kouri-Bairaktari, E. 2005: Evaluation of HER2 gene status in breast cancer by chromogenic in situ hybridization: comparison with immunohistochemistry Anticancer Research 25(2a): 939-946
Sunpaweravong, P.; Suwiwat, S.; Sunpaweravong, S.; Puttawibul, P.; Mitarnun, W. 2009: Correlation of epidermal growth factor receptor mutation, immunohistochemistry, and fluorescence in situ hybridization in esophageal squamous cell carcinoma Journal of the Medical Association of Thailand 92(9): 1136-1142
Mahdy, E.; Yoshihiro, S.; Zech, L.; Wester, K.; Pan, Y.; Busch, C.; Döhner, H.; Kallioniemi, O.; Bergerheim, U.; Malmström, P.U. 1999: Comparison of comparative genomic hybridization, fluorescence in situ hybridization and flow cytometry in urinary bladder cancer Anticancer Research 19(1a): 7-12
Penault-Llorca, F.; Jacquemier, J.; Le Doussal, V.; Voigt, J.J. 1999: Quality challenge for immunohistochemistry: example of the ERBB-2 status in breast cancer. Group for Evaluation of Prognostic Factors in Immunohistochemistry in Breast Cancer (GEFPICS) Annales de Pathologie 19(4): 280-282
Komminoth, P.; Adams, V.; Long, A.A.; Roth, J.; Saremaslani, P.; Flury, R.; Schmid, M.; Heitz, P.U. 1994: Comparison of histology, immunohistochemistry, RT-PCR, in situ hybridization, and in situ RT-PCR for demonstration of hepatitis C virus in paraffin-embedded liver biopsies Verhandlungen der Deutschen Gesellschaft für Pathologie 78: 220-225
Uemura, Y.; Kobayashi, M.; Muneishi, H.; Urata, T.; Hakoda, E.; Tanaka, Y.; Miyoshi, I. 1995: Genetic analysis by fluorescence in situ hybridization of lung cancer cells obtained by bronchial brushing Nihon Kyobu Shikkan Gakkai Zasshi 33(10): 1052-1057
Soguero Serrano, C. 1996: Immunohistochemistry and in situ hybridization in the identification of the hepatitis C virus in liver tissue Gastroenterologia y Hepatologia 19(5): 262-273
Yang, S.; Wang, M.; You, W. 1995: Overexpression of c-myc and p53 gene in human hepato-cellular carcinoma--a study with immunohistochemistry and in situ hybridization Zhonghua Zhong Liu Za Zhi 17(6): 415-417
Wu, G.D.; Shintaku, I.P.; Chien, K.; Geller, S.A. 1989: A comparison of routine light microscopy, immunohistochemistry, and in situ hybridization for the detection of cytomegalovirus in gastrointestinal biopsies American Journal of Gastroenterology 84(12): 1517-1520
Sarasophona, S.; Promsawat, K.; Rerkamnuaychok, B. 2000: Identification of sex chromosome by fluorescence in situ hybridization Journal of the Medical Association of Thailand 83(Suppl 1): S89-S94
Hove, M.G.; Hightower, B.J.; Graves, K. 2000: Use of combining in situ hybridization and immunohistochemistry in detecting human papillomavirus on routine sections in cases of diagnostic uncertainty Central African Journal of Medicine 46(8): 217-221
Hou, J.W.; Tsai, W.Y.; Wang, T.R. 1999: Detection of KAL-1 gene deletion with fluorescence in situ hybridization Journal of the Formosan Medical Association 98(6): 448-451
Multani, A.S.; Hopwood, V.L.; Pathak, S. 1996: A modified fluorescence in situ hybridization (FISH) technique Anticancer Research 16(6b): 3435-3437
Huang, Y.; Sun, X.; Li, Q. 1999: Diagnosis of sex chromosome abnormality by fluorescence in-situ hybridization Zhonghua Yi Xue Za Zhi 79(2): 106-108
Fernández, A.; Reigosa, A. 2013: Molecular classification of breast cancer patients obtained through the technique of chromogenic in situ hybridization (CISH) Investigacion Clinica 54(4): 406-416
Selig, S.; Okumura, K.; Ward, D.C.; Cedar, H. 1992: Delineation of DNA replication time zones by fluorescence in situ hybridization EMBO Journal 11(3): 1217-1225
Cheng, L.; Bucana, C.D.; Wei, Q. 1996: Fluorescence in situ hybridization method for measuring transfection efficiency Biotechniques 21(3): 486-491
Fiche, M. 2001: Fluorescence in situ hybridization (FISH): current developments and prospects in pathology Annales de Pathologie 21(5): 383-385