The dose-response relationship study between the quantitative morphological stereology on thyroid and different iodine doses in mice
Gao, Q.; Zhang, S.; Xu, C.; Liu, Y.; Wang, P.; Zhang, S.
Zhonghua Yu Fang Yi Xue Za Zhi 36(1): 38-40
2002
ISSN/ISBN: 0253-9624 PMID: 11955347 Document Number: 547587
Objective To study the dose-response relationship between the quantitative morphological stereology on thyroid and different iodine doses in mice. Methods Weaning Kunming mice were randomly divided into seven groups. The mice were fed for 100 days with distilled water containing different KIO3 concentrations, i. e. 50, 250, 500, 1 000, 1 500, 2 000, and 3 000 mug/L respectively. The 50 mug/L (proper idoine concentration) group was control group, and the groups of 250 apprx 3 000 mug/L were high iodine groups. The stereology parameters of thyroid follicle and follicular cavities were measured with HPIAS-1000 (High Resolution Pathological Image and word Analysis System). The stereology parameters included mean surface, volume on area, volume on circumference, specific surface, numerical density on area, spherical factor, the percentage of mean surface and mean volume of the follicular epithelial cell in thyroid follicle was further calculated. Results Positive correlations was observed between the thyroid absolute and relative weight, goiter rate and different iodine doses. And the thyroid absolute and relative weight of mice in the 250 mug/L group was significantly different from that in 50 mug/L group. The goiter rate of mice in different high iodine groups was in conformity with that of epidemiological investigation. The goiter rate of mice in 500 mug/L group was different from that in 50 mug/L group. Positive correlations were observed between mean surface, volume on area, volume on circumference, spherical factor and iodine doses, but the negative correlations were observed between numerical density on area, specific surface, the percentage of mean surface and mean volume of the follicular epithelial cell in thyroid follicle and iodine doses. Conclusions When Iodine doses are between 250 apprx 3 000 mug/L, the dose-response relationship was observed between the morphological stereology parameters of thyroid follicle and follicular cavities and iodine doses, and when the dose of iodine is 250 mug/L, it is possible to induce colloid goiter of mice. The goiter rates of mice resulted by different high iodine doses were in conformity with that of the epidemiological investigation of people.