Comparative study of catheter-mediated gene transfer into heart
Li, J.; Li, G.; Huang, C.; Jiang, H.; Tang, Q.; Xu, J.; Xia, H.; Wang, J.
Chinese Medical Journal 115(4): 612-613
2002
ISSN/ISBN: 0366-6999 PMID: 12133310 Document Number: 547481
Objective: To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). Methods: Fifteen dogs were used, and 0.3 ml (1X109 pfu) of an adenovirus (Adex1SR LacZ) was injected into the heart by 3 methods. The dogs were killed 5 days following injection, and gene expressions in heart and liver were evaluated by histochemical analysis. Results: The results showed that 1. the CAP method was relatively less damaging and induced sparse LacZ expression in the myocardium, and the gene expression was also found in both vessels within the myocardium and liver; 2. gene transfer by DMI resulted in intense LacZ expression around the injection accompanied by a local inflammatory response; 3. LacZ expression elicited by ICI was detected in either the inner surface of the parietal pericardium or epicardial surface of the heart, and also in the myocardium underlying the visceral pericardium. Conclusion: Three catheter-mediated methods of gene transfer into the heart may be used and a reasonable approach should be chosen according to purpose.