Development of a rat C6 brain tumor model
Zhang, X.; Wu, J.; Gao, D.; Fei, Z.; Qu, Y.; Jing, J.
Chinese Medical Journal 115(3): 455-457
2002
ISSN/ISBN: 0366-6999 PMID: 11940388 Document Number: 547397
Objective To establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume. Methods C6 cell suspension (10 mul) containing 10 g/L agarose and 1 X 106 cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains. Results Fifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%. Conclusion A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.