A novel immunization schedule for obtaining anti-LPS Haemophilus influenzae type b antibodies
Falcón Márquez, R.; Toraño Peraza, G.; Victoria Martínez, B.; Tamargo Martínez, I.
Revista Cubana de Medicina Tropical 53(1): 66-68
2001
ISSN/ISBN: 0375-0760 PMID: 11826543 Document Number: 532942
A novel immunization schedule was tested to increase antibodies to Haemophilus influenzae type B in which a 0.5% formaldehyde-inactivated cellular antigen and a partially purified lipopolysacharid antigen obtained from a selective precipitation with organic solvents were combined as immunogens. High specificity and biological activity antibodies with titers up to 1:256 were obtained by counterinmunoelectrophoresis; they were purified through a selective precipitation with ammonium sulphate and an ion-exchange chromatography using a DEAE-Sapharose column. The method proposed in this paper facilitates the production of purified antibodies useful for the improvement of serological diagnosis and H. influenzae b strain serotyping techniques.