Activation of NF-kappa B induced by stretching and the gene expression of beta-MHC regulated by NF-kappa B in cardiac myocytes
Liu, R.; Yin, H.; Wang, Z.
Hua Xi Yi Ke da Xue Xue Bao 32(3): 335-337
2001
ISSN/ISBN: 0257-7712 PMID: 12536555 Document Number: 532836
To determine the mechanism of reexpression of beta-MHC gene caused by the stimulation of stretch. The specimens were taken from the 1-, 2- and 3-day old SD rats. Immunohistochemical method was applied to observe the activation of NF-kappa B in stretched cardiac myocytes cultured on the silicon membrane, and then with the system of image analysis, the beta-MHC protein content was detected in four groups where NF-kappa B was activated or not activated, respectively. NF-kappa B was activated significantly by stretching cardiac myocytes for five hours. The beta-MHC protein content in stretched myocytes increased significantly, compared with that in the control myocytes, NAC-treated myocytes, and stretch-treated myocytes in the presence of NAC. The above data demonstrate that NF-kappa B signaling system is involved in regulating the stretch-induced gene expression of beta-MHC.