Determination and purification of beta-carotene in Spirulina maximum
Hu, Z.H.; Liu, Z.L.
Se Pu 19(1): 85-87
2001
ISSN/ISBN: 1000-8713 PMID: 12541855 Document Number: 529235
There are several kinds of nutritious materials in Spirulina maximum, and beta-carotene is one of the most important materials. Because beta-carotene is of low polarity, for purification it was extracted with the mixture of acetone and methanol in the ratio of 7:2 (V/V), then saponified, and extracted with petroleum ether, purified with column packed with neutral Al2O3. Its content was determined by reversed-phase HPLC. This method is accurate and the results are reproducible. The average recovery was 96.8%.