The effect of lactate-buffered peritoneal dialysis fluid in human peritoneal mesothelial cells function
Duan, S.; Liu, F.; Xiao, P.; Xia, J.; Peng, Y.; Ye, Y.; Luo, J.
Zhonghua Nei Ke Za Zhi 40(7): 463-466
2001
ISSN/ISBN: 0578-1426 PMID: 11798616 Document Number: 528564
To investigate the effect of lactate-buffered peritoneal dialysis fluid (L-PDF) on human peritoneal mesothelial cells (HPMC) function. Mesothelial cells from human omentum were isolated and cultured in vitro. Proliferation of HPMC was detected by MTT assay. The quantity of interleukin-8 (IL-8) and fibronectin (FN) in culture supernatants was determined by ELISA. Reverse transcription and polymerase chain reaction amplification were used to detect the expression of IL-8 mRNA in HPMC. Total cellular protein in these supernatants was estimated by using Lowry method. L-PDF inhibited proliferation of HPMC in a time-dependent manner. The levels of IL-8 and FN in the supernatants were remarkably high, and expression of IL-8 mRNA was up-regulated in HPMC exposure to L-PDF. During a subsequent recovery period of 48 hours in culture medium containing lipopolysaccharide (LPS, 10 mg/L), enterotoxin of staphylococcus aureus (10 mg/L) or TNFalpha(10 x 10(6) U/L), the levels of FN in the supernatants increased (P < 0.05). The levels of IL-8 also increased in HPMC stimulated with TNFalpha(P < 0.01). L-PDF inhibited proliferation of HPMC and increased levels of IL-8 and FN in the HPMC.