Preparation of Der p 1 specific monoclonal antibodies and use in a two-site-ELISA to detect Der p 1 allergen
Chien, T.I.; Chen, Y.G.; Chiang, B.L.
Journal of Microbiology Immunology and Infection 33(2): 87-92
2000
ISSN/ISBN: 1684-1182 PMID: 10917877 Document Number: 524500
It has been well documented that the mite Dermatophagoides pteronyssinus (Der p) is the major allergen in house dust in Taiwan. The purpose of this study is to develop a sandwich immunometric assay which can be used for the standardization of mite allergen extracts or house dust, for assessing the procedures of mite avoidance, and for identifying species in epidemiological studies. Der p 1 allergen (both recombinant and native Der p 1) was purified and a panel of Der p 1 specific monoclonal antibodies was developed. The monoclonal antibodies were biotinylated and a two-site ELISA with peroxidase-conjugated streptavidin was developed for the detection of Der p 1 allergen. It is suggested that these monoclonal antibodies could be applied both in affinity columns for the purification of native Der p 1 antigen and in sandwich-ELISA for epidemiological study.