Measurement of macrophage colony-stimulating factor during in-vitro fertilization and embryo transfer cycles
Quan, S.; Wan, L.; Song, L.
Zhonghua Fu Chan Ke Za Zhi 35(1): 42-44
2000
ISSN/ISBN: 0529-567X PMID: 11831950 Document Number: 523508
To explore the influences of macrophage colony-stimulating factor (M-CSF) on follicle development, ovulation, fertilization and embryo quality during in-vitro fertilization and embryo transfer (IVF-ET) cycles. 36 patients underwent 54 cycles of controlled ovarian hyperstimulation by gonadotropin-releasing hormone agonist-human menopausal goudotropin-human chorionic gonadotropin for IVF-ET program. M-CSF concentrations in serum were measured at early follicular phase, mid-follicular phase and the day of oocyte retrieval by enzyme-linked immunosorbent assay. Follicular fluid (FF) on the day of egg retrieval was also measured for M-CSF concentration. M-CSF concentration in serum gradually increased throughout ovarian hyperstimulation and reached a peak on the day of oocyte retrieval. M-CSF concentration in FF was significantly higher than that in serum on the same day (P < 0.01). The M-CSF concentration in FF from follicles where oocytes could be retrieved, fertilized or with a diameter > or 16 mm, volume > or 2 ml, was significantly higher than those from follicles where oocyte could not be retrieved, fertilized or with a diameter < 16 mm, volume < 2 ml, respectively (P < 0.01). However, there was no significant difference in M-CSF concentration of FF between high and low embryo grade groups (P > 0.05). M-CSF may play an important role as an intraovarian regulator in the process of follicle development, maturation and ovulation. Therefore, M-CSF may exert an effect on fertilization process but not on the embryo quality.