Isolation of emetine resistant clones of Entamoeba histolytica by petri dish agar method
Prabhu, R.; Sehgal, R.; Chakraborti, A.; Malla, N.; Ganguly, N.K.; Mahajan, R.C.
Indian Journal of Medical Research 111: 11-13
2000
ISSN/ISBN: 0019-5340 PMID: 10793488 Document Number: 519118
Emetine-resistant clones of Entamoeba histolytica strain HM1: IMSS were isolated by using the petri dish agar method after mutation with ethyl-methanesulphonate. Two emetine-resistant clones were obtained; both were resistant to emetine at a concentration of 24 micro g/ml. The 50% inhibitory concentration (IC50) was 5 micro g/ml for emetine-sensitive clones and 14 micro g/ml for the resistant clones. The colony forming efficiency of E. histolytica strain HM1: IMSS varied from 44 to 54%. It is concluded that this method is useful for isolating clones from different strains of the parasite for molecular and immunological studies.