In vitro expression of proalpha1 (I) collagen mRNA by human pre-osteoclastic cells
Masi, L.; Malentacchi, C.; Benvenuti, S.; Amedei, A.; Bigozzi, U.; Montali, E.; Brandi, M.L.
Journal of Endocrinological Investigation 23(1): 1-4
2000
ISSN/ISBN: 0391-4097 PMID: 10698043 Document Number: 518628
In vitro studies have demonstrated that the extracellular matrix modulates the cell phenotype. In the present study we have investigated in vitro proalpha1(I) collagen mRNA expression in a human pre-osteoclastic cell line (FLG 29.1 cells) in basal condition and after various stimuli. In addition, in order to evaluate the effect of cell-cell interactions on collagen type I mRNA expression, we have cultured the human pre-osteoclastic cells FLG 29.1 with either the human osteoblast-like cell line Saos-2 or the bovine bone endothelial cell line BBE. We showed that the FLG 29.1 cells express proalpha1(I) collagen mRNA, whose expression is modulated by phorbol esters (TPA). Co-culturing FLG 29.1 cells with either Saos-2 or BBE cells induced decrease of proalpha1(I) collagen mRNA expression.