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Development of the insulin-like growth factor-i assay system

Mine, T.; Yano, I.; Tatsumi, N.; Terano, Y.; Yamamoto, K.; Goda, T.; Kosaka, Y.; Hishida, K.

Osaka City Medical Journal 46(2): 129-144

2000

ISSN/ISBN: 0030-6096
PMID: 11252732
Document Number: 518295
A high speed full automatic ELISA system for measurement of insulin-like growth factor-I (IGF-I) was established by using magnetic particle-linked monoclonal antibody and enzyme-labeled monoclonal antibody. A standard curve was obtained, and the effect of dilution on the assay system was investigated. An IGF-I spike recovery test of human serum samples and a study of the correlation with a radioimmunoassay system were performed, and good results were obtained from all studies. The assay range was 0.5-50 ng/ml, and the time required for the full automatic measurement was 15 minutes. This assay system will play a central role in the clinical approach to IGF-I.

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