Detection of a human parvovirus intrauterine infection with the polymerase chain reaction
Dong, Z.W.; Zhou, S.Y.; Li, Y.; Liu, R.M.
Journal of Reproductive Medicine 45(5): 410-412
2000
ISSN/ISBN: 0024-7758 PMID: 10845175 Document Number: 517246
OBJECTIVE: To detect human parvovirus B19 intrauterine infection in pregnancy with the polymerase chain reaction (PCR). STUDY DESIGN: DNA of chorionic villi and amniotic fluid was extracted and the gene of human parvovirus B19 amplified with PCR. RESULTS: The study analyzed 61 specimens of chorionic villi and 26 specimens of amniotic fluid and found two positive specimens of chorionic villi and 1 positive specimen of amniotic fluid. CONCLUSION: The vertical transmission of human parvovirus B19 infection in early pregnancy may be a pathway of intrauterine infection. Chorionic villus sampling in early pregnancy and PCR could be developed as a method of prenatal diagnosis of human parvovirus B19 intrauterine infection.