Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA
Saito, S.; Yamauchi, H.; Hasui, Y.; Kurashige, J.; Ochi, H.; Yoshida, K.
Research Communications in Molecular Pathology and Pharmacology 107(1-2): 39-44
2000
ISSN/ISBN: 1078-0297 PMID: 11334369 Document Number: 515048
In order to establish a quantitative assay for 8-OHdG concentrations in urine, we examined the precision of a test for the recovery of 8-OHdG in urine by using an ELISA method. The coefficient of variation (CV) for assay with incubation in water or air ranged between 7.0% and 8.4% and between 19.2% and 30.6%, respectively. The data by using incubation in water gave higher accuracy than those in air. The recovery rates of 8-OHdG in urine sample ranged from 95 - 114%. Our results indicated that the excellent sensitivity of this ELISA kit by using incubation in water makes its use possible for the determination in urine with a good reproducibility and recovery of 8-OHdG-spiked samples.