Gas chromatographic analysis of fosfomycin in plasma for pharmacokinetic analysis
Webster, G.K.; Bell, R.G.
Journal - Association of Official Analytical Chemists 82(3): 620-624
1999
ISSN/ISBN: 0004-5756 PMID: 10367380 Document Number: 509300
An efficient method for gas chromatographic analysis of fosfomycin in plasma was developed for preliminary investigations of the bioavailability in poultry of 3 commercial complexes of fosfomycin: a levorotatory Ca(-) salt, a racemic Ca(+/-) salt, and a tromethamine (THAM) salt. The method was used to determine whether the less expensive racemic mixture would provide equivalent levels of fosfomycin in blood as the pure Ca(-) form and the THAM salt. The THAM salt, a more expensive product to market, was thought to have the greatest bioavailability. The assay is selective, sensitive, and applicable to pharmacokinetic analysis.