A PCR-aided transcript titration assay (PATTY) to measure topoisomerase i gene expression in human tumor specimens
Meersma, G.J.; Bakker, M.; Groen, H.J.; Van der Zee, A.G.; Jensen, P.B.; Giaccone, G.; De Vries, E.G.; Smit, E.F.
Anticancer Research 19(2a): 1309-1315
1999
ISSN/ISBN: 0250-7005 PMID: 10368697 Document Number: 505217
Twenty-seven Citrus flavonoids were examined for their activity of induction of terminal differentiation of human promyelocytic leukemia cells (HL-60) by nitro blue tetrazolium (NBT) reducing, nonspecific esterase, specific esterase, and phagocytic activities. 10 flavonoids were judged to be active (percentage of NBT reducing cells was more than 40% at a concentration of 40 microM), and the rank order of potency was natsudaidain, luteolin, tangeretin, quercetin, apigenin, 3, 3, '4, '5, 6, 7, 8-heptamethoxyflavone, nobiletin, acacetin, eriodictyol, and taxifolin. These flavonoids exerted their activity in a dose-dependent manner. HL-60 cells treated with these flavonoids differentiated into mature monocyte/macrophage. The structure-activity relationship established from comparison between flavones and flavanones revealed that ortho-catechol moiety in ring B and C2-C3 double bond had an important role for induction of differentiation of HL-60. In polymethoxylated flavones, hydroxyl group at C3 and methoxyl group at C8 enhanced the differentiation-inducing activity.
Document emailed within 1 workday
