Complex grabbing
Sikorski, R.; Peters, R.
Science 285(5435): 1868
1999
ISSN/ISBN: 0036-8075 PMID: 10515792 Document Number: 502926
The writers discuss a clever method for isolating extremely pure complexes of proteins from cell cultures that can be used for protein sequencing. Developed by a German team led by G. Riguat and reported in a forthcoming Nature Biotechnology, the procedure is known as tandem affinity purification and involves attaching multiple affinity tags to a protein, which can then be used in its isolation.
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