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Stability of normal and hyperacetylated chromatin in the cell free system of Drosophila embryos

Kraevskiĭ, V.A.

Molekuliarnaia Biologiia 33(5): 750-757

1999

ISSN/ISBN: 0026-8984
PMID: 10579178
Document Number: 500020
The stability of normal and hyperacetylated chromatin was studied in cell-free system of Drosophila embryos. Structural rearrangement of chromatin took place in this system, resulting in the release of a portion of nucleosomal DNA supercoils and increased chromatin sensitivity to DNAse. This rearrangement also supported a high level of nucleosomal DNA conformational mobility. It was shown that the structural rearrangement is much more effective in cases of hyperacetylated chromatin.

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Kogan, G.L.; Berman, A.E.; Mazurov, V.I. 1973: Collagen biosynthesis in a cell-free system in the presence of RNA isolated from the polysomes of chick embryos Biokhimiia 38(4): 872-874
Alvarez, M.R. 1973: Microfluorometric comparisons of chromatin thermal stability in situ between normal and neoplastic cells Cancer Research 33(4): 786-790
Malý, A.; Hradec, J.; Ríman, J. 1974: Chicken cell-free protein synthesizing system programmed by endogenous mRNA. Reaction conditions and comparison of normal and tumour systems Neoplasma 21(4): 395-400
Farmer, H.; Frazier, J.A. 1986: Presence of a togavirus-like agent in cell cultures from specific pathogen free chick embryos Veterinary Record 119(10): 243-244
Zador, E.; Budai, D. 1994: Expression of the acetylcholinesterase gene during development of drosophila embryos Neurobiology 2(4): 301-309
Baek, K.H.; Ambrosio, L. 1994: An efficient method for microinjection of mRNA into Drosophila embryos Biotechniques 17(6): 1024-1026
Gigliotti, S.; Rotoli, D.; Graziani, F.; Malva, C. 2003: Oogenesis in Drosophila melanogaster: a model system for studying cell differentiation and development Italian Journal of Biochemistry 52(2): 104-111
Paddock, S.W.; Langeland, J.A.; DeVries, P.J.; Carroll, S.B. 1993: Three-color immunofluorescence imaging of Drosophila embryos by laser scanning confocal microscopy Biotechniques 14(1): 42-48
Zykova, T.Y.; Demidova, D.S.; Kokoza, E.B.; Zhimulev, I.F. 2016: Localization of Mobile P-Element Insertions in Domains of Active and Repressed Chromatin in Drosophila Melanogaster's Genome Tsitologiia 58(4): 258-261
Subbotina, N.A. 1978: Comparative study of the effect of different rifamycins on bacterial cell metabolism and on the RNA-polymerase reaction in a cell-free system Antibiotiki 23(3): 239-242
Lebedeva, L.I.; Fedorova, S.A.; Omel'ianchuk, L.V. 2003: Genetic control of mitosis. Premature beginning of telophase chromatin reorganization in cells of the Drosophila melanogaster strain with ff3 mutation Genetika 39(7): 939-946
Kolchinskiĭ, A.M.; Vashakidze, R.P.; Preobrazhenskaia, O.V.; Karpov, V.L.; Mirzabekov, A.D. 1984: Chromatin structure of ribosomal genes of Drosophila melanogaster. Random location of nucleosomes in DNA and characteristics of organization of non-transcribed spacer Molekuliarnaia Biologiia 18(4): 1141-1150
Ueda, T. 2004: Cell-free gene expression system Seikagaku. Journal of Japanese Biochemical Society 76(2): 130-134
Koen, I.M.; Zbarskiĭ, I.B. 1978: RNA transport from rat liver cell nuclei in vitro. Release of rapidly labeled RNA from isolated nuclei in a cell-free system in the presence of RNAse affectors Biokhimiia 43(12): 2115-2123
Petraşincu, D.E.; Szegli, G.; Mazîlu, E.; Gaicu, N.; Herold, A.; Roşu, A.; Chivu, L.; Haraga, L.; Stoean, I. 1987: Effect of oxygen free radicals on some genetic and cell growth parameters of human normal cells cultivated in vitro Archives Roumaines de Pathologie Experimentales et de Microbiologie 46(3): 241-266
Maianskiĭ, A.N.; Iskhakova, S.K.; Molchanova, I.V. 1976: Immunoreactivity to the products of normal microflora. I. Observations on stability of the level of normal human antibodies to common antigens of enterobacteria Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 7: 65-67
Ding, R.; Smulson, M. 1994: Depletion of nuclear poly(ADP-ribose) polymerase by antisense RNA expression: influences on genomic stability, chromatin organization, and carcinogen cytotoxicity Cancer Research 54(17): 4627-4634
Szybiak, U.; Legocki, A.B. 1983: Control of protein synthesis in a wheat germ cell-free system Acta Biochimica Polonica 30(3-4): 255-263
Lisenkov, A.F.; Babushkina, L.M.; Gnedoĭ, S.N.; Khil'ko, S.N. 1981: Expression of plasmid R6K in a cell-free coupled transcription-translation system Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1981(2): 23-27
Gorkina, N.V.; Mazurov, V.I. 1972: Different degrees of inhibition by chloramphenicol of protein synthesis in a cell-free system Voprosy Meditsinskoi Khimii 18(1): 104-107