Isotachophoresis analysis of hydrolytic products of DFP catalyzed by DFPase in porcine liver
Huang, Z.; Ma, L.; Liu, W.; Cheng, Y.
Se Pu 17(2): 196-198
1999
ISSN/ISBN: 1000-8713 PMID: 12549168 Document Number: 499755
DFPase can hydrolyze the P-F bond of diisopropyl phosphate fluoride (DFP) to produce diisopropyl phosphate and HF. In this article, the activity of two kinds of DFPase from porcine liver was assayed by the methods of titration and F- ion sensitive electrode. The results showed that the activity of large molecular DFPase can be determined by both methods, but the activity of small molecular DFPase can only be determined by the titration and cannot be determined by F- ion sensitive electrode. The isotachophoresis analysis of the products of DFP hydrolyzed by the large and small molecular DFPase was studied. The results showed that the small molecular enzyme could catalyze the hydrolysis of DFP, but without the production of F- ion, and that this enzyme is one kind of phosphoesterase that hydrolyzed P-OR bond of DFP.