A study on PCR for detecting infection with M. leprae
Qinxue, W.; Xinyu, L.; Wei, H.; Tao, L.; Yaoping, Y.; Jinping, Z.; Xiuling, C.; Ganyun, Y.
Chinese Medical Sciences Journal 14(4): 237-241
1999
ISSN/ISBN: 1001-9294 PMID: 12894900 Document Number: 498419
So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point. Based on the M. leprae gene coding groEL, 65 kD and 16S rRNA, three polymerase chain reactions were developed by using Plikaytis', Woods' and Pattyn's procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose. For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practical purpose, the Woods' PCR optimized by us ought to be chosen firstly.