A new system for expressing heterologous gene in Escherichia coli regulated by oxygen consistence in the environment
Tong, Q.; Yang, S.; Gong, Y.
Chinese Journal of Biotechnology 15(3): 177-182
1999
ISSN/ISBN: 1042-749X PMID: 10935165 Document Number: 498292
The expression of Vitreosilla hemoglobin gene (vgb) is regulated by oxygen consistence in E. coli. The gene transcription is activated under the condition of limited oxygen. A new system for expressing heterologous gene in E. coli regulated by dissolved oxygen consistence was constructed. It includes a host bacteria GJ100, which contained T7 RNA polymerase gene controlled by vgb promoter, and an expression vector on which the heterologous gene was under the control of a T7 promoter. The results indicated that E. coli thioredoxin A, IgG binding domain of Staphylococcus protein A(ZZ), snake neurotoxin, salmon calcitonin hexa-polymer, human interleukinII (IL2) and human pro-urokinase genes could be expressed efficiently. Expression level was more than 30% of the total cellular protein.