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Comparative genomic hybridization: technical development and cytogenetic aspects for routine use in clinical laboratories

Lapierre, J.M.; Cacheux, V.; Da Silva, F.; Collot, N.; Hervy, N.; Wiss, J.; Tachdjian, G.

Annales de Genetique 41(1): 56-62

1998

ISSN/ISBN: 0003-3995
PMID: 9599653
Document Number: 496549
Comparative genomic hybridization (CGH) offers a new global approach for detection of chromosomal material imbalances of the entiere genome in a single experiment without cell culture. In this paper, we discuss the technical development and the cytogenetic aspects of CGH in a clinical laboratory. Based only on the visual inspection of CGH metaphase spreads, the correct identification of numerical and structural anomalies are reported. No commercial image analysis software was required in these experiments. We have demonstrated that this new technology can be set up easily for routine use in a clinical cytogenetics laboratory.

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