Functional analysis of nuclear hormone receptors
Nagaya, T.; Seo, H.
Nihon Rinsho. Japanese Journal of Clinical Medicine 56(7): 1688-1692
1998
ISSN/ISBN: 0047-1852 PMID: 9702038 Document Number: 496256
Nuclear hormone receptors are transcription factors which regulate gene transcription by lipophilic hormones. The functional domains such as transactivation, DNA binding, ligand binding and dimerization are mapped in the distinct region of the receptors. To analyze these functions, ligand binding assay, gel shift assay, transfection study, GST pull-down method, immunoprecipitation method, two hybrid system and yeast functional assay are employed. These assays have been shown to clarify the molecular basis of the disorders which are due to mutation in the receptors.