Changes of the interleukin-6 levels in skin at different sites after thermal injury
Kawakami, M.; Terai, C.; Okada, Y.
Journal of Trauma 44(6): 1056-1063
1998
ISSN/ISBN: 0022-5282 PMID: 9637162 Document Number: 495628
Objective: To delineate the regulation of IL-6 production in unburned skin adjacent to a burn in an animal model. Methods: In C57BL/6 mice, at 15, 30, and 60 minutes after a 20% full-thickness burn, skin was removed from various sites. Control samples were obtained from unburned mice. Normal skins were incubated with tumor necrosis factor-alpha(TNF-alpha), interleukin-1 alpha(IL-1alpha), IL-1 beta, and IL-6. Unburned skin specimens were incubated with anti-TNF-alpha and IL-lot antibodies. Cytokine levels were measured by enzyme-linked immunosorbent assay. Results: The burn increased the IL-6 levels at 30 minutes (p < 0.05) and the IL-1alpha levels at 15 and 60 minutes in the unburned skin. TNF-alpha, IL-1alpha, and IL-1beta increased IL-6 production in normal skin (p < 0.05). Anti-IL-1alpha antibody decreased IL-6 production in the unburned skin (p < 0.05). Conclusions: IL-1alpha modulates IL-6 production in unburned skin after injury. IL-6 and IL-1alpha might contribute to the alterations after a burn.