Colonization of Anopheles pseudopunctipennis from Mexico
Villarreal, C.; Arredondo-Jiménez, J.I.; Rodriguez, M.H.; Ulloa, A.
Journal of the American Mosquito Control Association 14(4): 369-372
1998
ISSN/ISBN: 8756-971X PMID: 10084128 Document Number: 495491
Two colonies of Anopheles pseudopunctipennis, Tapachula and Abasolo strains, were established under laboratory conditions with a thermoperiod (29 degrees C during the day; 24 degrees C during the night) and artificial dusk. To stimulate mating, a light beam from a flashlight was shone on the cage shortly after lights off. This procedure was repeated for the first 6 mosquito generations (parental to F6) and thereafter light stimulation was unnecessary for mating. The Tapachula colony has been maintained for 24 generations in 24 months, with insemination rates in females > 80% since the F3, and a monthly production of 30,000 pupae since the F7. Using the same procedure, the Abasolo colony from northeastern Mexico has been maintained for 13 generations in 14 months, with insemination rates of 26-52%.
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