Effects of mibefradil, a blocker of T-type Ca2+ channels, in single myocytes and intact muscle of guinea-pig heart
Emanuel, K.; Mackiewicz, U.; Pytkowski, B.; Lewartowski, B.
Journal of Physiology and Pharmacology An Official Journal of the Polish Physiological Society 49(4): 577-590
1998
ISSN/ISBN: 0867-5910 PMID: 10069698 Document Number: 489867
We investigated the effects of a relatively selective blocker of the T-type Ca2+ channels, mibefradil (MBF), in the isovolumic left ventricles of the isolated, perfused hearts of guinea-pigs and single myocytes isolated from the ventricles of this species. In the myocytes superfused with 0 Na+ solution containing 200 microM lidocaine and pulsed from -90 mV to -40 mV to +5 mV, MBF proved to be about 3 times more potent inhibitor of the T-type than of the L-type Ca2+ current. The effect on the L-type current was strongly voltage and use dependent. In the ventricles and in the myocytes contraction was reduced by 50% by about 1 microM MBF, the concentration 12 times higher than this increasing the coronary flow by 50%. In myocytes the decrease in unloaded shortening paralleled inhibition of the T-type rather, than of the L-type Ca2+ current. Inhibition of electrically stimulated contraction of the myocytes was three times stronger than inhibition of the caffeine contractures regarded as an index of sarcoplasmic reticulum (SR) Ca2+ content. These findings are consistent with the hypothesis that the T-type Ca2+ channels may contribute to release of Ca2+ from the SR. It is concluded that MBF has a definite negative inotropic effect in the ventricular myocardium of guinea-pig heart at the concentrations found in the blood of the patients submitted to the clinical trials.