Morphologic studies on the biologic seal of titanium dental implants. Report I. In vitro study on the epithelialization mechanism around the dental implant
Kawahara, H.; Kawahara, D.; Hashimoto, K.; Takashima, Y.; Ong, J.L.
International Journal of Oral and Maxillofacial Implants 13(4): 457-464
1998
ISSN/ISBN: 0882-2786 PMID: 9714951 Document Number: 489812
To propose a mechanism for apical epithelialization at the implant-tissue interface, cell contact to titanium surfaces and adhesive strength of epithelial-like (HGE) and fibroblastic (HGF) cells derived from human gingiva were investigated under three different media conditions containing plaque extracts: nonfiltered, 5-micron pore filtered, and 0.22-micron pore filtered. The plaque extracts had a greater effect in decreasing the growth rate of the HGF than of the HGE. Similarly, the HGE exhibited greater adhesive strength than the HGF. These differences in the cells' resistance to plaque extracts were also observed using light and electron microscopy. Evidence from this study suggests that the difference in growth, contact, and adhesive strength of the HGE and HGF cells to titanium surfaces may promote apical epithelialization under the pathologic condition.