Neutralizing human antibody specific for human cytomegalovirus in E. coli expression system
Ihara, S.; Maeda, F.; Takekoshi, M.
Nihon Rinsho. Japanese Journal of Clinical Medicine 56(1): 161-166
1998
ISSN/ISBN: 0047-1852 PMID: 9465683 Document Number: 488621
We isolated neutralizing human Fab fragment specific for human cytomegalovirus (HCMV) by phage display system. Fab libraries were constructed from peripheral lymphocyte of healthy individual. In several clones reacted for HCMV-infected HEL cells, one clone, designated 13-3, stained HCMV infected cells at 96 hrs post infection. It didn't react to cells at 6 hrs post infection or infected cells in the presence of AraC, meaning that 13-3 recognized protein synthesized at late times of infection. It also neutralized HCMV, Towne strain, efficiently. This neutralizing activity was specific for HCMV and no effect for HSV-1 and 2.