Use of a fluorescent method for detection of lymphocyte Fc-receptors
Shiauris, A.I.; Miliukene, V.V.; Peshkus, I.K.
Ukrainskii Biokhimicheskii Zhurnal 70(1): 121-125
1998
ISSN/ISBN: 0201-8470 PMID: 9848153 Document Number: 488210
FcR on chicken blood lymphocytes have been studied by fluorimetric binding assay. Binding data were analyzed by Scatchard's method. Temperature and time variations were investigated in order to optimise the binding conditions. It was concluded that binding FcR to IgG on chicken blood lymphocytes was optimal at 0 degree C for 60 min. 10-14% of nonspecific binding were detected in different experiments. Scatchard binding plots at 0 degree C demonstrated that IgG bound to FcR per cell 2.3 +/- 4 x 10(5) with affinity 3.9 +/- 0.5 x 10(5) M-1.