Separation of human bladder cancer cells from bone marrow with a kind of immunomagnetic microspheres
Kang, J.; Samten, B.; Xie, S.; Wei, S.
Yao Xue Xue Bao 33(1): 52-56
1998
ISSN/ISBN: 0513-4870 PMID: 11938936 Document Number: 487516
By binding a monoclonal antibody BDI-1 to polystyrene magnetic micospheres using physical and chemical methods together, a kind of immunomagnetic microspheres (IMMS), which can specifically bind target cells and give it magnetic response, was sucessfully constituted. The results of indirect immunofluorescence and cell binding suggest that IMMS can effectively bind target cells while do not have non-specific binding activity. The influence of antibody added and the ratio between IMMS and cells were also studied. After purging cancer cells from bone marrow of mice with IMMS, the cancer cells were effectively removed while most of the bone marrow cells were recovered.