Modulation of adhesion and chemotaxis of human neutrophils by cortisol, transforming growth factor-beta and antiinflammatory drugs
Zak-Nejmark, T.; Jankowska, R.; Malolepszy, J.; Kraus-Filarska, M.; Nadobna, G.; Nowak, I.A.
Journal of Investigational Allergology and Clinical Immunology 8(6): 346-352
1998
ISSN/ISBN: 1018-9068 PMID: 10028481 Document Number: 485279
Neutrophils are the first cells that accumulate in response to stimuli from the arising inflammation site. We have studied the influence of commonly used antiinflammatory (acetylsalicylic acid) and immunosuppressive (cyclosporin A, methotrexate) drugs and endogenous substances of antiinflammatory activity (cortisol, transforming growth factor-beta; TGF-beta) on the interdependent phenomena of cell motility and adhesion. Neutrophils from healthy subjects were preincubated with various concentrations of these substances and adhesion to plastic-bound monoclonal antibodies to CD11a/CD18, CD11c/ CD18 and CD44 was determined. We have found that the studied drugs significantly lowered adhesion (by 40%) of neutrophils to the ligands. Simultaneously, cell motility was investigated according to the Boyden method. We found a significant dose-dependent increase in the motility of this cells in a range comparable to conventional chemoattractants for neutrophils. These data indicate that simultaneous enhancement of motility and reduction of adhesion might be a common pathway for the mechanisms of action of the most common anti-inflammatory drugs.