Recombinant DNA technique and sickle cell anemia research
Thomas, K.R.; Capecchi, M.R.
Science 275(5305): 1404-1405
1997
ISSN/ISBN: 0036-8075 PMID: 9072801 Document Number: 483369
A letter responds to Cole-Strauss et al.'s September 6, 1996, report regarding the application of a recombinant DNA technique to sickle-cell anemia research. The data in the report infer that, after exposure to oligonucleotides containing wild-type bA sequences, 50-80 percent of mutant, bS-globin loci in a B-cell population were converted to wild-type alleles. The absolute recombination frequency represented by this remarkable phenomenon is 3-6 orders of magnitude greater than that normally observed in cultured mammalian cells. A reply is published.