Substrates and inhibitors of human T-cell leukemia virus type 1 (HTLV-1) proteinase
Hrusková-Heidingsfeldová, O.; Bláha, I.; Urban, J.; Strop, P.; Pichová, I.
Leukemia 11(Suppl 3): 45-46
1997
ISSN/ISBN: 0887-6924 PMID: 9209292 Document Number: 482656
Human T-cell leukemia virus type 1 (HTLV-1) proteinase, a 125 residue polypeptide, was chemically synthesized using the solid phase method. The crude product was purified, renaturated and proteolytic activity was tested using oligopeptide substrates derived from processing sites of various retroviral polyproteins. Cleavage of the oligopeptide substrates together with an initial study using a series of HIV-1 and MAV (myeloblastosis associated virus) proteinase inhibitors suggest that the substrate specificity of HTLV-1 proteinase is very close to that of BLV (bovine leukemia virus) proteinase and distinct from that of both HIV-1 and MAV proteinases.