Detection and differentiation of Helicobacter pylori from gastric biopsy and saliva by PCR-SSCP
Zhang, Y.; Lu, X.
Zhonghua Nei Ke Za Zhi 36(7): 446-449
1997
ISSN/ISBN: 0578-1426 PMID: 10436943 Document Number: 481500
In this study we detected the species-specific antigen gene of Helicobacter pylori (Hp) from gastric biopsy specimens and saliva of 48 patients by polymerase chain reaction (PCR), then adopted single strand conformation polymorphism (SSCP) to differentiate Hp strains from population and different sites of the same individual. Among patients of positive gastric biopsy results, the detection rate of saliva was 72.2% (26/36). In 12 patients of negative gastric biopsy results, 2 saliva specimens were positive. Further SSCP analysis demonstrated that in population the infected Hp strains showed 6 different band patterns. SSCP analysis of PCR products from the gastric biopsy specimen and saliva of the same individual showed no significant difference. The results showed that Hp at different sites of one individual probably belongs to the same strain.