Amplification of hypervariable genomic regions for establishment of the type of hematopoiesis in hemoblastosis patients after allogeneic bone marrow transplantation
Demidova, I.A.; Surin, V.L.; Mendeleeva, L.P.; Savchenko, V.G.
Genetika 33(4): 546-549
1997
ISSN/ISBN: 0016-6758 PMID: 9206670 Document Number: 480217
To establish the type of hemopoiesis in 15 patients with hemoblastosis subjected to allogenic bone marrow transplantation, the amplification of four hypervariable human genome loci containing tandem repeats with varying copy numbers (loci ApoB, DX, S52, VWF, and YNZ22) were studied by means of polymerase chain reaction. The sensitivity determined by amplification of DNA mixture in dilutions was 1-2%. Based on the data obtained, various types of hemopoiesis recovery after bone marrow transplantation were determined; in most cases, a complete donor chimerism was revealed; in some patients, mixed chimerism; and in one case, a host type of hemopoiesis was found. An association between hemopoiesis type and further development of the disease was observed.