Characterization of the oligosaccharide component of arylsulfatase B from rat liver
Przybyło, M.; Lityńska, A.
Acta Biochimica Polonica 44(2): 181-190
1997
ISSN/ISBN: 0001-527X PMID: 9360706 Document Number: 476736
A glyean chain analysis of the total oligosaccharide pool derived from rat liver arylsulfatase B was carried out by P4 Gel Permeation Chromatography and sequential exoglycosidase digestion. It was found that 71% of rat liver arylsulfatase B oligosaccharides were sialylated. The relative contribution of particular structures in the total glycan pool was as follows: sialylated biantennary complex type glycans with terminal galactose - 65%, high-mannose type glycans - 15%, biantennary complex type glycans with core fucose and terminal N-acetylglucosamine - 5%, O-linked oligosaccharides - 3.5%.