Evolution of a phosphorothioate RNA library during in vitro selection
Kawakami, J.; Sugimoto, N.
Nucleic Acids Symposium Series 1997(37): 201-202
1997
ISSN/ISBN: 0261-3166 PMID: 9586069 Document Number: 476513
We carried out an in vitro selection of aptamers from an RNA library with phosphorothioate linkages instead of normal phosphodiesters. This "thio-RNA" library had smaller dissociation rate constant (kd) to a target protein than a natural RNA library. On the basis of the results, an improved selection procedure to obtain ideal nucleic acid drugs will be discussed.
Document emailed within 1 workday
Related Documents
Dubrovskaia, V.V.; Ulitin, A.B.; Laman, A.G.; Gileva, I.P.; Bormotov, N.I.; Il'ichev, A.A.; Brovko, F.A.; Shchelkunov, S.N.; Belanov, E.F.; Tikunova, N.V. 2007: Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus Molekuliarnaia Biologiia 41(1): 173-185Goodnight, C.J.; Schwartz, J.M.; Stevens, L. 1992: Contextual analysis of models of group selection, soft selection, hard selection, and the evolution of altruism American Naturalist 140(5): 743-761
Tamura, N.; Iwatani, W.; Shoji, Y.; Shimada, J.; Mizushima, Y. 1995: Aptameric inhibition of in vitro DNA polymerization by phosphorothioate oligonucleotides Nucleic Acids Symposium Series 34: 93-94
Hnatowich, D.J.; Mardirossian, G.; Fogarasi, M.; Sano, T.; Smith, C.L.; Cantor, C.R.; Rusckowski, M.; Winnard, P. 1996: Comparative properties of a technetium-99m-labeled single-stranded natural DNA and a phosphorothioate derivative in vitro and in mice Journal of Pharmacology and Experimental Therapeutics 276(1): 326-334
Pizer, I.H.; Franz, D.R.; Brodman, E. 1963: Mechanization of library procedures in the medium-sized medical library. I. The serial record Bulletin of the Medical Library Association 51: 313-338
Womboh, B.S.H. 1993: The library and information resources of the University of Agriculture, Makurdi Library, Nigeria: a self-study Quarterly Bulletin of the International Association of Agricultural Information Specialists 38(1): 9-15
Krsiak, M. 2006: Human evolution: humanistic selection and looking to the future Neuro Endocrinology Letters 27(5): 563-567
Capittini, C.; Martinetti, M.; Cuccia, M. 2008: MHC variation, mate choice and natural selection: the scent of evolution Rivista di Biologia 101(3): 463-480
Zhang, Y-xin.; Liu, Y.; Liu, K-tai.; Cheng, J.; Chen, X-hua.; Wang, G.; Wang, G-quan. 2003: Construction of subtracted cDNA library in human Jurkat T cell line induced by arsenic trioxide in vitro Zhonghua Yu Fang Yi Xue Za Zhi 37(6): 403-407
Vannucci, L.; Frigenti, L.; Faussone-Pellegrini, M-Simonetta. 2011: From conception to birth: ancient library sources of embryology and women anatomy kept in the Biblioteca Biomedica of the Università degli Studi di Firenze (Biomedical Library of Florence University) Italian Journal of Anatomy and Embryology 116(2): 93-103
Li, J.; Hong, W. 2014: Feature extraction for breast cancer data based on geometric algebra theory and feature selection using differential evolution Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 31(6): 1218-22 1228
Campagnoli, C.; Di Gregorio, A.; Arisio, R.; Fessia, L. 1985: Patient selection for in vitro fertilization (IVF) and embryo transfer (ET) Experientia 41(12): 1491-1494
Zhenodarova, S.M.; Kliagina, V.P. 1998: New classes of ribozymes isolated by in vitro selection. RNA ligases Molekuliarnaia Biologiia 32(6): 948-954
Nishikawa, F.; Chiba, A.; Shirai, M.; Fauzi, H.; Taira, K.; Kumar, P.K.; Nishikawa, S. 1995: In vitro selection analysis of trans-acting HDV ribozyme Nucleic Acids Symposium Series 1995(34): 115-116
Kumm, J.; Laland, K.N.; Feldman, M.W. 1994: Gene-culture coevolution and sex ratios: the effects of infanticide, sex-selective abortion, sex selection, and sex-biased parental investment on the evolution of sex ratios Theoretical Population Biology 46(3): 249-278
Sacchi, A.; Corsi, A.; Caputo, M.; Zupi, G. 1979: In vitro and in vivo selection of two Lewis lung carcinoma cell lines Tumori 65(6): 657-664
Zebrowska, J.Irena. 2010: In vitro selection in resistance breeding of strawberry (Fragaria x ananassa duch.) Communications in Agricultural and Applied Biological Sciences 75(4): 699-704
Bennici, A. 2005: A new paradigma on the plant evolution: from a natural evolution to an artificial evolution? Rivista di Biologia 98(1): 39-46
Sciola, L.; Spano, A.; Pippia, P.; Barni, S. 1993: Various nuclear and cytoplasmic parameters in relation to the evolution of an in vitro culture of rat fibroblasts Bollettino della Societa Italiana di Biologia Sperimentale 69(4): 215-222
Oe, Y.; Ikawa, Y.; Shiraishi, H.; Inoue, T. 2000: Analysis of the P7 region within the catalytic core of the Tetrahymena ribozyme by employing in vitro selection Nucleic Acids Symposium Series 2000(44): 197-198