Effect of chlorfenvinphos on rat liver subjected to ischemia and reperfusion
Kamiński, M.; Wiaderkiewicz, R.; Siekierska, E.
Przeglad Lekarski 54(10): 693-701
1997
ISSN/ISBN: 0033-2240 PMID: 9478088 Document Number: 473474
This study was performed on 9 groups of rats. Groups 1-3 served as controls. In groups 4 and 5 rat livers were subjected to 90-min ischaemia followed by 12- or 24-h reperfusion. In groups 6 and 7 rats were injected with intraperitoneal chlorfenvinphos (2 mg/kg b.w.) and killed after 12 or 24 h. In groups 8 and 9 rat livers were subjected to 90-min ischaemia, 12- or 24-h reperfusion and then rats were injected with chlorfenvinphos (2 mg/kg b.w.). Liver sections were evaluated morphologically, histochemically (SDH, LDH, G6Pase, glycogen, Mg2+ ATPase and AcP). The microsomal fraction of the liver was evaluated for cytochrome P450 content and NADPH-cytochrome P-450 reductase activity. It was found that liver ischaemia and reperfusion resulted in extensive necrosis, enzymatic disturbances, particularly in acinar zone 3. Ischaemia as well as reperfusion decreased the cytochrome P450 content of hepatocytic microsomes and the activity of NADPH-cytochrome P-450 reductase. Intraperitoneal injection of chlorfenvinphos during ischaemia and reperfusion dramatically intensified damage to the liver, although chlorfenvinphos alone produced only mild nonspecific effects on the morphological and enzymatic structure of the liver.