Analysis of interaction of human RNA-polymerase Ii and TATA-binding protein
Sokolenko, A.A.; Sedova, V.M.; Tulokhonov, I.I.; Savinkova, L.K.
Molekuliarnaia Biologiia 31(6): 962-965
1997
ISSN/ISBN: 0026-8984 PMID: 9480431 Document Number: 473383
Preincubation of human RNA polymerase II with 32P-oligonucleotides containing the TATA element of the promoter of the human beta-actin gene followed by incubation with the TATA-binding protein in increasing concentrations leads to a proportional drop in the amount of complexes of RNA polymerase II with the oligonucleotides. Preincubation of RNA polymerase II with increasing concentrations of TATA-binding protein followed by incubation with equal amounts of a phosphoramide derivative of 32P-oligonucleotide containing a TATA element showed that as the TATA-binding protein concentration increases, the amount of covalent complexes of RNA polymerase II with the oligonucleotide derivative diminishes. These results suggest displacement of TATA-containing oligonucleotides by the TATA-binding protein from complexes with RNA polymerase.