Top Abstract Purchase Related
Home
>
Node
>
Subnode

Malignant transformation of human prostate epithelial cells by N-nitroso-N-methylurea

Rhim, J.S.; Jin, S.; Jung, M.; Thraves, P.J.; Kuettel, M.R.; Webber, M.M.; Hukku, B.

Cancer Research 57(4): 576-580

1997

ISSN/ISBN: 0008-5472
PMID: 9044828
Document Number: 473028
We report the malignant transformation of adult human prostate epithelial cells after multiple exposures to the chemical carcinogen N-nitroso-N-methylurea. Such transformants showed morphological alterations and anchorage-independent growth in soft agar and induced carcinomas when transplanted into nude mice. No p53 or ras mutations were observed. Stepwise chromosomal changes in the progression to tumorigenicity were observed. Loss of the p arms of chromosome 8 (p10 gt pter) and chromosome 10 (p10 gt pter) and gain of the q arm of chromosome 8 (q10 gt qter) were only observed in the tumor outgrows. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to a chemical carcinogen.

Document emailed within 1 workday
Secure & encrypted payments

Zhu, L.-j.; Zhang, M.; Ju, L. 2009: Malignant transformation of human bronchial epithelial cells induced by man-made mineral fibers Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 27(4): 234-235
Yang, M.; Xu, J.-n.; Wang, Q.-k.; Li, Y.; Sun, J.-x.; Li, J.-y.; Xie, G.-y.; Wang, J. 2009: Study on malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate Zhonghua Yu Fang Yi Xue Za Zhi 43(3): 187-192
Kaighn, M.E.; Reddel, R.R.; Lechner, J.F.; Peehl, D.M.; Camalier, R.F.; Brash, D.E.; Saffiotti, U.; Harris, C.C. 1989: Transformation of human neonatal prostate epithelial cells by strontium phosphate transfection with a plasmid containing SV40 early region genes Cancer Research 49(11): 3050-3056
Sun, H.; Gong, Y.; Wang, Q.; Chen, J. 1999: Malignant transformation of HPV-18 immortalized human bronchial epithelial cells induced by tobacco-specific nitrosamine NNK Wei Sheng Yan Jiu 28(2): 95-96
Likhachev, A.J.; Ivanov, M.N.; Brésil, H.; Planche-Martel, G.; Montesano, R.; Margison, G.P. 1983: Carcinogenicity of single doses of N-nitroso-N-methylurea and N-nitroso-N-ethylurea in Syrian golden hamsters and the persistence of alkylated purines in the DNA of various tissues Cancer Research 43(2): 829-833
Liu, H.; Wang, Q.; Xie, G.; Li, H.; Wang, A.; Wen, Y.; Xu, J. 2015: Anti-oncogene of opioid binding protein/cell adhesion molecule-like methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 33(11): 812-815
Jiang, Y.; Chen, J.; Chen, X. 2001: Malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene metabolite dihydroxyepoxy benzo pyrene Wei Sheng Yan Jiu 30(3): 129-131
Marquardt, H.; Heidelberger, C. 1972: Influence of "feeder cells" and inducers and inhibition of microsomal mixed-function oxidases on hydrocarbon-induced malignant transformation of cells derived from C3H mouse prostate Cancer Research 32(4): 721-725
Feng, F.; Zhang, Q.; Zhou, F.; Wu, Y.; Wu, Y. 2014: Tumor necrosis factor-α and NF-κB play a role in macrophage-like THP-1 cells promoting coal tar pitch extract-induced tumorigenic transformation of human bronchial epithelial cells Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 32(1): 38-43
Yarita, T.; Nettesheim, P.; Williams, M.L. 1978: Tumor induction in the trachea of hamsters with N-nitroso-N-methylurea Cancer Research 38(6): 1667-1676
Geder, L.; Sanford, E.J.; Rohner, T.J.; Rapp, F. 1977: Cytomegalovirus and cancer of the prostate: in vitro transformation of human cells Cancer Treatment Reports 61(2): 139-146
Harris, C.C.; Brash, D.E.; Lechner, J.F.; Mark, G. 1988: Aberrations of growth and differentiation pathways during neoplastic transformation of human epithelial cells Iarc Scientific Publications 1988(92): 139-148
Milo, G.E.; Noyes, I.; Donahoe, J.; Weisbrode, S. 1981: Neoplastic transformation of human epithelial cells in vitro after exposure to chemical carcinogens Cancer Research 41(12 Part 1): 5096-5102
Yu, D.-J.; Tang, Y.-Q.; Shi, Y.-F.; Wang, Y.-C.; Zhuo, J.; Zhu, Y.-Y.; Sun, X.-W.; Xia, S.-J. 2010: Proportion of intermediate epithelial cells and human prostate cancer Zhonghua Nan Ke Xue 16(12): 1063-1067
Seidel, G.; Fey, F. 1979: Studies of the target cell problem in N-nitroso-N-methylurea induced leukemogenesis Acta Biologica et Medica Germanica 38(10): 1461-1465
Bernard, D.J.; Maurizis, J.C.; Chassagne, J.; Sauvezie, B.; Fonck, Y.; Chollet, P.; Plagne, R. 1990: Effects of cyclosporine a on Ia antigen expression in N-nitroso-N-methylurea-induced rat mammary tumors Cancer Research 50(11): 3301-3305
Paraskeva, C.; Corfield, A.P.; Harper, S.; Hague, A.; Audcent, K.; Williams, A.C. 1990: Colorectal carcinogenesis: sequential steps in the in vitro immortalization and transformation of human colonic epithelial cells (review) Anticancer Research 10(5a): 1189-1200
Gullino, P.M.; Grantham, F.H.; Hill, D.M.; Pettigrew, H.M. 1985: Effect of pregnancy and nursing on the growth of metastases from N-nitroso-N-methylurea-induced mammary carcinomas Journal of the National Cancer Institute 74(1): 229-234
Eliseev, V.V.; Vlasov, N.N. 1980: Mammary gland tumor induction in rats by N-nitroso-N-methylurea and N-methyl-N1-nitro-N-nitrosoguanidine Voprosy Onkologii 26(2): 62-65
Taylor-Papadimitriou, J.; Burchell, J.; Hurst, J. 1981: Production of fibronectin by normal and malignant human mammary epithelial cells Cancer Research 41(6): 2491-2500