Polymerase chain reaction in the detection of patients infected by Chlamydia trachomatis after treatment
Wang, H.; Wang, J.; Liu, Y.
Zhonghua Yi Xue Za Zhi 77(2): 91-93
1997
ISSN/ISBN: 0376-2491 PMID: 9596935 Document Number: 472393
To investigate the value of polymerase chain reaction (PCR) for follow-up patients infected by Chlamydia trachomatis. Follow-up specimens were collected from 30 patients. Chlamydia trachomatis positive were detected by PCR and direct fluorescence assay test (DFA) in the 30 patients before therapy. 15 patients were treated with minocycline (100 mg twice daily) for 10 days, and 15 patients were treated with 1.0 g of azithromycine as a single oral dose. After 1-2 weeks of antimicrobial therapy, all patients had negative DFA for Chlamydia trachomatis, but 9 had positive Chlamydia trachomatis DNA as detected by PCR. The 9 specimens were not confirmed to livae viable organisms of Chlamydia trachomatis. The debris of nonviable Chlamydia trachomatis DNA was excluded from urinogenital tract at about one month.