Characterization of satellite cells derived from chickens with the low score normal (LSN) muscle weakness
Li, Z.; Velleman, S.G.; McFarland, D.C.; Pesall, J.E.; Gilkerson, K.K.; Ferrin, N.H.; Yun, Y.
Cytobios 91(365): 75-85
1997
ISSN/ISBN: 0011-4529 PMID: 9589604 Document Number: 470190
Myogenic satellite cell clones were established from the pectoralis major muscle of chickens with the low score normal (LSN) muscle weakness, and from controls. The percentage of cells which attached to substrata and began to proliferate was higher for the control line than the LSN line (55% vs 30%). Of the clones which initiated growth, 63% of the control cells and only 32% of the LSN cells proliferated to confluence in 25 cmsuperscript 2 tissue culture flasks. Proliferation rates were significantly lower with LSN satellite cells than with controls. LSN satellite cells were less responsive to the mitogenic effects of chicken serum and differentiation rates were lower compared with controls. There was a greater number of insulin-like growth factor (IGF) receptors on LSN satellite cells compared with controls. The IGF receptor binding affinities between the two cell lines were similar. The results suggest that a defect in satellite cell physiology may contribute to the skeletal muscle weakness seen in the LSN line.