Studies on the transfer techniques of three maize genes
Wang, G.; Zhang, H.; Ding, Q.; Xie, Y.; Dai, J.
Chinese Journal of Biotechnology 12(1): 53-60
1996
ISSN/ISBN: 1042-749X PMID: 8877115 Document Number: 469316
Maize transformation has been carried out through microprojectile bombardment, ultrasonication in a DNA buffer, and ovary-injection with a self-made microinjector. The plasmid pB48.415, which carries a 3'-truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with a particle gun, embryogenic calli ultrasonicated under different conditions of ovaries injected 10-20 hours after pollination. The results of Dot blotting and Southern blotting analyses proved the integration of the Bt gene into maize genome.