Alterations in brain tumor DNA detected by a fingerprinting probe
Joshi, A.R.; Sinha, S.; Dil-Afroz; Sulaiman, I.M.; Banerji, A.K.; Hasnain, S.E.
Indian Journal of Biochemistry and Biophysics 33(6): 455-457
1996
ISSN/ISBN: 0301-1208 PMID: 9219429 Document Number: 467850
We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.