The sequences of intron 50 and 51 of DMD gene and the deletion mechanism of 51st exon
Sheng, W.; Chai, J.; Liu, Z.
Zhonghua Yi Xue Za Zhi 76(11): 852-854
1996
ISSN/ISBN: 0376-2491 PMID: 9275539 Document Number: 463772
To investigate the relationship between the structural characters of 50th and 51st introns and deletion mechanisms of 51st exon of DMD gene. We cloned the 3.1 Kb-Hind I human genome DNA fragment containing the 50 and 51 introns of DMD gene, sequenced the fragment completely, and analysed the character of the sequences with computer. The length of the fragment was 3179, including the entire 51st exon and partial 50th and 51st intron sequences. There were 36 short tandom repeats, 22 direct and inverted repeats, and 8 homologous sequences in the 50th and 51st introns. A TATA box was found in the intron 50 and it may be another new promotor of DMD gene. The hypothesis, which the recombination of repeat sequences may result in the deletion of 51st exon, is put forward.