Lectin-histochemical study of O-linked glycoconjugates in dysplastic retina of Norrie disease
Uehara, F.; Sameshima, M.; Yanagita, T.; Iwakiri, N.; Ohba, N.
Japanese Journal of Ophthalmology 40(2): 251-254
1996
ISSN/ISBN: 0021-5155 PMID: 8876395 Document Number: 463577
The carbohydrate chains of O-linked glycoconjugates in dysplastic retina from a Japanese female infant with Norrie disease were examined by lectin histochemistry. The avidin-biotinylated peroxidase method was used. The retina was highly dysplastic and composed of undifferentiated embryonic tissues containing a number of rosettes of varying sizes. The lumina of the rosettes were stained by peanut agglutinin, which recognizes the Gal beta 1,3GalNAc sequence of O-linked glycans. However, the lumina were not labeled by wheat germ agglutinin, which reacts with sialic acid and/or N-acetylglucosamine. These observations suggest that the O-linked glycoconjugates in the lumina of rosettes were not sialylated in the present case. Their lack of terminal sialic acids may be related to the rosette formation.